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![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Spatial deteil vs acceptance angle
Acceptance angle of objective must be wide enough to collect at least first order diffracted rays
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
See back focal plane using telescope
The rear focal plane of the objective can be observed using a telescope insert into the eyepiece tube.
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Diffraction pattern in the transform plane
Demonstration of diffraction patterns in a test object - (Diatom test slide).
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Spatial filtering in the transform plane
Using a small aperture slider to remove diffracted orders in the transform plane eliminates detail in the image plane.
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Deriving Numerical Aperture = N.A.
The geometries of diffraction patterns must match the geometries of acceptance angle.
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Condenser N.A. must at least match that of the objective to realize full resolution.
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Complex frequency-based information can be decomposed into combinations of simple waveforms.