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MMG195A Lecture 17 - Principles of Microscopy
Lecture 17 for MMG195A, Spring 2020 semester. By Dr. Menelaos Symeonides.
N.A Plus immersion oil
Using the 550 nM full wave compensator
Complex frequency-based information can be decomposed into combinations of simple waveforms.
Condenser N.A. must at least match that of the objective to realize full resolution.
Cell wall between crossed polars
Numerical Aperture vs useful magnification
A quick estimate of useful magnification: N.A. X 1000.
Spatial information is encoded in the diffracted rays.