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![](/wowza/users/m/s/msymeoni/thumbs/cJHFqQKpFXKVvqbPsmtL.jpg)
MMG195A Lecture 17 - Principles of Microscopy
Lecture 17 for MMG195A, Spring 2020 semester. By Dr. Menelaos Symeonides.
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Spatial deteil vs acceptance angle
Acceptance angle of objective must be wide enough to collect at least first order diffracted rays
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
See back focal plane using telescope
The rear focal plane of the objective can be observed using a telescope insert into the eyepiece tube.
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Diffraction pattern in the transform plane
Demonstration of diffraction patterns in a test object - (Diatom test slide).
![](https://streaming.uvm.edu/cc-content/themes/overcast/assets/file-audio-regular.png)
Spatial filtering in the transform plane
Using a small aperture slider to remove diffracted orders in the transform plane eliminates detail in the image plane.