MMG195A Lecture 17 - Principles of Microscopy

By: msymeoni

Lecture 17 for MMG195A, Spring 2020 semester. By Dr. Menelaos Symeonides.

Parfocality

By: plintilh

Parfocal lenses

Numerical aperture revisited

By: plintilh

N.A Plus immersion oil

Using the 550 nM full wave compensator

By: plintilh

red plate

Fourier musical analogy

By: plintilh

Complex frequency-based information can be decomposed into combinations of simple waveforms.

Importance of Condenser N.A.

By: plintilh

Condenser N.A. must at least match that of the objective to realize full resolution.

Cell wall between crossed polars

By: plintilh

between polars

Numerical Aperture vs useful magnification

By: plintilh

A quick estimate of useful magnification: N.A. X 1000.

Zero order has no information

By: plintilh

Spatial information is encoded in the diffracted rays.